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Tuesday, January 26 • 11:00am - 11:20am
Fusobacteria as a Marker to Estimate the Abundance of Asian Carp and Total Fish Population In Illinois River

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AUTHORS: Wen-Tso Liu*, Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Lin Ye, State Key Laboratory of Pollution Control and Resource Reuse, School of The Environment, Nanjing University, Camila Carlos, Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Ya Zhang, Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Takashi Narihiro, Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, and Masaru Nobu, Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Andrew F. Casper, Illinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana-Champaign, Jon Amberg, Upper Midwest Environmental Sciences Center, United States Geological Survey Mark Gaikowski, Upper Midwest Environmental Sciences Center, United States Geological Survey

ABSTRACT: To develop a genetic-based method for Asian carp and total fish surveillance, this study characterized the bacterial community in the guts of 129 fish (17 fish species in total) caught from different water bodies in the U.S. using 16S rRNA gene sequence as the biomarker. By comparing with the microbiota in other animal guts, including human, beef cattle, chicken, goose, swine, and dairy cattle, it was found that the phylum Fusobacteria is almost unique to freshwater fish. Further analysis showed that the majority of the Fusobacteria (>90% for most fish) in fish guts mainly affiliated two clusters under the genera of Cetobacterium and Hados.Sed.Eubac.3, respectively. In addition, a Leptotrichia-related and a Cetobactrium-related cluster were found to be unique to bigmouth buffalo and silver carp, respectively. Based on these findings and the fact that Fusobacteria members are obligate anaerobic bacteria and cannot grow in river water, we have designed and developed a series of PCR primers for a microbial source tracking method, and are currently validating the specificity of these primers with known samples. In addition, we will apply these primers to estimate and correlate the abundance of Asian carp and total fish in water bodies with samples from different location along Illinois River.

Tuesday January 26, 2016 11:00am - 11:20am EST
Pantlind

Attendees (6)