Midwest Fish & Wildlife Conference 2016

AUTHORS: Leah Cronan*, Lucigen Corporation; Chris Merkes, U.S. Geological Survey, Upper Midwest Environmental Sciences Center; Chris Rees, U.S. Fish and Wildlife Service; Jon Amberg, U.S. Geological Survey, Upper Midwest Environmental Sciences Center; Thomas Schoenfeld, Lucigen Corporation

ABSTRACT: Early detection is critical to preventing the spread of invasive species. Environmental DNA (eDNA) provides a means of identifying invasive species when direct visual detection is not feasible. However, current methods for detecting eDNA are confined to central labs. Sample transport and reporting time and availability of specialized personnel can delay results and limit the utility of molecular detection in preventing spread. To provide a field-compatible tool for detecting eDNA, Lucigen has partnered with UMESC to develop a portable, easy-to-use, one hour test for bighead and silver carp eDNA. The test is based on isothermal amplification of mitochondrial DNA markers unique to these two species and detects as little as 100 DNA copies with specificity against co-occurring species. eDNA samples are collected by filtration using a battery powered peristaltic pump. Further sample preparation is incorporated into the amplification step and no additional DNA extraction steps are required before introducing the sample to the test mix. It has been formatted for easy use in field conditions lacking access to electrical power or specialized equipment. The test provides actionable results to users with very limited training or experience performing and interpreting molecular tests. The test is formatted for stable storage at room temperature, eliminating the need for cold chain storage and handling and uses available battery-powered instruments that provide simple positive/negative answers in the field to the minimally trained user and log data for detailed analysis at a later date by experts. Performance data from lab studies will be provided.